Hudson Institute accesses world class specialist mass spectrometry and proteomic equipment and expertise though a subscription with the Bio21 Proteomics Facility.
The facility currently has the following mass spectrometers
- 2 x Thermo NanoLC/ OrbiTrap Fusion Lumos
- 2 x Thermo NanoLC/ Q Exactive Plus mass spectrometer
- Thermo NanoLC/ OrbiTRAP ELITE ETD mass spectrometer
- Thermo Exactive Focus
- Agilent 6520 LC/ESI-TOF mass spectrometer
- Waters Vion IMS QTOF mass spectrometer
- Waters Synapt HDX G2-Si Ion mobility mass spectrometer
- Shimadzu 9030 QTOF LCMSMS
- 4 x Agilent HPLC systems for sample preparation.
- 3 x Shimadzu Prep HPLC with UV and MS detection
- Waters M Class HPLC for micro-purification
This equipment and downstream analysis can be used to perform common and more complex methods such as
- In-solution protein identification and IP-MS identification
- In-gel protein identification
- Characterise intact recombinant proteins by mass
- Confirm chemical synthesis
- Identify phosphorylation sites
- Immune-precipitation of protein complexes
- Labelled and label-free quantitation
- Stable Isotope Labelling by/with Amino acids in Cell culture
- APEX biotin labelling
- Hydrogen Deuterium Exchange
Contact us
Dr Daniel Gough
e: daniel.gough@hudson.org.au
Dr Daniel Garama
Proteomics Specialist
e: daniel.garama@hudson.org.au