Proteomics

Hudson Institute accesses world class specialist mass spectrometry and proteomic equipment and expertise though a subscription with the Bio21 Proteomics Facility.

The facility currently has the following mass spectrometers

  • 2 x Thermo NanoLC/ OrbiTrap Fusion Lumos
  • 2 x Thermo NanoLC/ Q Exactive Plus mass spectrometer
  • Thermo NanoLC/ OrbiTRAP ELITE ETD mass spectrometer
  • Thermo Exactive Focus
  • Agilent 6520 LC/ESI-TOF mass spectrometer
  • Waters Vion IMS QTOF mass spectrometer
  • Waters Synapt HDX G2-Si Ion mobility mass spectrometer
  • Shimadzu 9030 QTOF LCMSMS
  • 4 x Agilent HPLC systems for sample preparation.
  • 3 x Shimadzu Prep HPLC with UV and MS detection
  • Waters M Class HPLC for micro-purification

This equipment and downstream analysis can be used to perform common and more complex methods such as

  • In-solution protein identification and IP-MS identification
  • In-gel protein identification
  • Characterise intact recombinant proteins by mass
  • Confirm chemical synthesis
  • Identify phosphorylation sites
  • Immune-precipitation of protein complexes
  • Labelled and label-free quantitation
  • Stable Isotope Labelling by/with Amino acids in Cell culture
  • APEX biotin labelling
  • Hydrogen Deuterium Exchange

Contact us

Dr Daniel Gough
e: daniel.gough@hudson.org.au

Dr Daniel Garama
Proteomics Specialist
e: daniel.garama@hudson.org.au