Dr Duncan Crombie, Children’s Cancer Foundation Senior Organoid Specialist

In the collaborative Hudson Monash Paediatric Precision Medicine Program, scientists at Hudson Institute will establish a living biobank of paediatric brain tumours and solid cancers – including living organoids or lab-grown ‘mini-tumours’ – to trial and develop targeted treatments and improve clinical outcomes, survival rates and quality of life (limiting side-effects) for childhood cancer patients. As the Senior Organoid Specialist for the program, Duncan oversees the culture of tumour cells in the laboratory and uses an automated platform to screen for compounds that inhibit tumour growth, providing both insight into cancer cell biology and an avenue for identifying potential therapeutics.

Duncan brings extensive knowledge of cell culture systems and automation, gained from research positions across academic institutions and industry, to support the activities of the Program. Furthermore, Duncan’s knowledge of pluripotent stem cell biology and in vitro disease modelling also provides for a different perspective in understanding paediatric tumour biology. He is also a current member of the Australasian Society for Stem Cell Research and the International Society for Stem Cell Research.

Selected publications

  • Daniszewski M, Nguyen Q, Chy HS, Singh V, Crombie DE, Kulkarni T, Liang HH, Sivakumaran P, Lidgerwood GE, Hernández D, Conquest A, Rooney LA, Chevalier S, Andersen SB, Senabouth A, Vickers JC, Mackey DA, Craig JE, Laslett AL, Hewitt AW, Powell JE, Pébay A (2018) Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media. iScience 7:30-39.

  • Daniszewski MS, Pébay A, Crombie DE (2017) Benefits of automation for pluripotent stem cell therapies, disease modeling & drug discovery. Cell and Gene Therapy Insights 3:613-621.

  • Crombie DE, Curl CL, Raaijmakers AJ, Sivakumaran P, Kulkarni T, Wong RC, Minami I, Evans-Galea MV, Lim SY, Delbridge L, Corben LA, Dottori M, Nakatsuji N, Trounce IA, Hewitt AW, Delatycki MB, Pera MF, Pébay A (2017) Friedreich’s ataxia induced pluripotent stem cell-derived cardiomyocytes display electrophysiological abnormalities and calcium handling deficiency. Aging (Albany NY) 9:1440-1452.

  • Crombie DE, Daniszewski M, Liang HH, Kulkarni T, Li F, Lidgerwood GE, Conquest A, Hernández D, Hung SS, Gill KP, De Smit E, Kearns LS, Clarke L, Sluch VM, Chamling X, Zack DJ, Wong RCB, Hewitt AW, Pébay A (2017) Development of a Modular Automated System for Maintenance and Differentiation of Adherent Human Pluripotent Stem Cells. SLAS Discov 22:1016-1025.

  • Crombie DE, Pera MF, Delatycki MB, Pébay A (2016) Using human pluripotent stem cells to study Friedreich ataxia cardiomyopathy. Int J Cardiol 212:37-43.

  • Crombie DE, Van Bergen N, Davidson KC, Anjomani Virmouni S, Mckelvie PA, Chrysostomou V, Conquest A, Corben LA, Pook MA, Kulkarni T, Trounce IA, Pera MF, Delatycki MB, Pébay A (2015) Characterization of the retinal pigment epithelium in Friedreich ataxia. Biochem Biophys Rep 4:141-147.

  • Frisca F, Crombie DE, Dottori M, Goldshmit Y, Pébay A (2013) Rho/ROCK pathway is essential to the expansion, differentiation, and morphological rearrangements of  human neural stem/progenitor cells induced by lysophosphatidic acid. J Lipid Res 54:1192-1206.

  • Lim SY, Lee DG, Sivakumaran P, Crombie D, Slavin J, Dottori M, Conley B, Denham M, Leung J, Tee R, Dusting GJ, Pebay A, Dilley RJ (2012) In vivo tissue engineering chamber supports human induced pluripotent stem cell survival and rapid differentiation. Biochem Biophys Res Commun 422:75-79.

  • Croxford AM, Crombie D, McNaughton D, Holmdahl R, Nandakumar KS, Rowley MJ (2010) Specific antibody protection of the extracellular cartilage matrix against collagen antibody-induced damage. Arthritis Rheum 62:3374-3384.

  • Zaibak F, Bello P, Kozlovski J, Crombie D, Ang H, Dottori M, Williamson R (2009) Unrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres. BMC Biotechnol 9:101.

  • Crombie DE, Turer M, Zuasti BB, Wood B, McNaughton D, Nandakumar KS, Holmdahl R, Van Damme MP, Rowley MJ (2005) Destructive effects of murine arthritogenic antibodies to type II collagen on cartilage explants in vitro. Arthritis Res Ther 7:R927-937.

  • Crombie DE, Mackay IR, Wood BR, McNaughton D, Rowley MJ (2005) Images of interest. Hepatobiliary and pancreatic: Liver histopathology: Fourier transform infrared microspectroscopic imaging for objective and quantifiable assessment of liver biopsies. J Gastroenterol Hepatol 20:485.