Professor Caroline Gargett from the Endometrial Stem Cell Biology Research Group at Hudson Institute

Professor Caroline Gargett

Human endometrial epithelial progenitor cells

Professor Gargett (PhD 1997) is world-renowned for her discovery of stem/progenitor cells in human endometrium, the highly regenerative lining of the uterus, establishing a new field of research in reproductive biology. She formed the Endometrial Stem Cell Biology Group in 2004 following the award of the top scoring NHMRC New Investigator grant to study endometrial stem cell biology. Having since determined the identity of the endometrial epithelial progenitor cell (bases of glands) and mesenchymal stem/ stromal cell (around blood vessels) through her discovery of novel surface markers for each cell type, she is now applying these breakthroughs to common gynaecological disorders. Her group currently comprises 5 postdoctoral scientists, 3 support staff and 6 students.

She leads a multidisciplinary project on endometriosis in collaboration with Prof Grant Montgomery at University of Queensland funded by the US Department of Defense. This project aims to determine the role of endometriosis risk genes in endometrial epithelial stem/progenitor cell function using integrated single cells RNAseq and ATACseq, endometrial epithelial organoid and patient-derived xenograft technologies.

She leads an interdisciplinary project in collaboration with Monash Health urogynaecologists developing a cell-based therapy using endometrial mesenchymal stem/stromal cells and degradable nanobiomaterials to treat and prevent POP, a common disorder affecting 1 in 4 women. She has developed several sheep models of POP and vaginal birth injury to evaluate these new tissue engineering constructs as she works toward translating her team’s finding into the clinic.

With a strong record of research excellence and discovery, Professor Gargett has secured NHMRC funding since 2004, including 7 CIA grants, an RD Wright CDA (2007-11), Senior Research (2013-18) and Investigator (2020-2024) Fellowships. She has also secured SIEF funding for a prestigious John Stocker Postdoctoral Fellowship (for Dr Shayanti Mukherjee), and funding from the CASS Foundation, MIME, Australian Stem Cell Centre, Cancer Council Victoria, Therapeutic Innovations Australia, RANZCOG, Monash IVF Research Foundation and other philanthropic sources.

She is recipient of numerous national and international awards including the Society for Gynecological Investigation President’s Achievement Award (2013), the Endometriosis Foundation of America (2011), European Society for Human Reproduction and Embryology Established Scientist Award (2005), and the Society for Reproductive Biology’s RCRH Award for research excellence (2011). Professor Gargett’s research featured in the NHMRC’s 10 of the Best Research Projects in 2008 and 10 years later in 3 video clips on the NHMRC front webpage. She has received widespread publicity in Australia and internationally and is frequently invited to give presentations at international conferences.

Professor Gargett is a Board Member of the National Stem Cell Foundation of Australia (since 2015) and Stem Cells Ltd (since 2019), and a member of the Scientific Advisory Board of the Centre for Commercialisation of Regenerative Medicine Australia node (since 2018). She holds editorial board positions on Scientific Reports, Biology of Reproduction, Reproductive Sciences (2009-2019) and Fertility and Sterility (2011-14). She is an Associate Editor for Reproductive Sciences (and Human Reproduction (2005-8). She was President of the Australasian Society for Stem Cell Research (2013-2014) where she led the successful bid to host the International Society for Stem Cell Research Annual meeting in Melbourne in 2018. She has served as Secretary for the Society for Reproductive Biology (2005-7) and currently on the International Urogynecology Association Basic Science SIG Steering Committee.

Pelvic Organ Prolapse is not a sexy topic but it should be. Pelvic organ prolapse is a condition that affects one in five of us. It’s the reason we work so hard on our pelvic floor exercises during pregnancy. But one in five women will still need surgery to fix it.

In ABC’s Babytalk podcast, Professor Caroline Gargett National talks about the damage that can occur during childbirth, the options women have and the opportunities for new treatments.

Endometriosis is not a sexy topic either, despite one in ten young women having the condition. Endometriosis takes up to 10 years to diagnose, and causes severe pelvic pain and infertility. There is no cure and it often recurs after surgery.

In this explainer in The Conversation, Professor Caroline Gargett and her team write about why it takes so long for endometriosis to be diagnosed.

Selected publications

  • Lucciola R, Vrljicak P, Gurung S, Filby C, Darzi S, Muter J, Ott S, Brosens JJ, Gargett CE (2020) Impact of sustained transforming growth factor-β receptor inhibition on chromatin accessibility and gene expression in cultured human endometrial MSC. Front Cell Dev Biol 8:567610

  • Emmerson S, Mukherjee S, Melendez J, Cousins F, Edwards SL, Karkalainen P, Ng M, Tan, KS, Darzi S, Bhakoo K, Rosamilia A, Werkmeister JA, Gargett CE. (2019) Composite mesh design for delivery of autologous mesenchymal stem cells influences mesh integration, exposure and biocompatibility in an ovine model of pelvic organ prolapse. Biomaterials 225: 119495. (IF 10.273; 8 citations)

  • Mukherjee S, Darzi S, Rosamilia A, Kadam V, Truong Y, Werkmeister JA, Gargett CE (2019) Blended nanostructured degradable mesh with Endometrial Stem Cells promote Tissue and anti-inflammatory response in vivo for pelvic floor application. Biomacromolecules 20: 454-468. (12 citations)

  • Nguyen H, Xiao L, Deane JA, Tan KS, Cousins FL, Masuda H, Sprung CM, Rosamilia A, Gargett CE (2017) N-cadherin identifies human endometrial epithelial progenitor cells by in vitro stem cell assays. Human Reproduction 32:2254-2268. (35 citations)

  • Gargett CE, Schwab KE, Deane JA (2016) Endometrial Stem/progenitor cells: the first 10 years. Human Reproduction Update 22:137-163 (IF11.194; >275 citations)

  • Gargett CE, Schwab KE, Puttemans P, Brosens JJ, Benagiano G, Brosens I (2014) Potential role of endometrial stem/progenitor cells in the pathogenesis of early-onset endometriosis. Molecular Human Reproduction 20:591-598 (135 citations)

  • Ulrich D, Edwards SL, Su C, Tan KS, White JF, Ramshaw JAM, Lo C, Rosamilia A, Werkmeister JA. Gargett CE (2014) Human endometrial mesenchymal stem cells modulate the tissue response and mechanical behavior of polyamide mesh implants for pelvic organ prolapse repair. Tissue Engineering Part A 20:785-798 Featured on the Front Cover 14.39 Oct 7, 2013 (>95 citations)

  • Masuda H, Anwar S, Bühring HJ, Rao J, Gargett CE (2012) A novel marker of human endometrial mesenchymal stem-like cells. Cell Transplantation 21: 2201-2214 (>190 citations)

  • Gargett CE, Schwab KE, Zillwood RM, Nguyen HPT, Wu D (2009) Isolation and culture of epithelial progenitors and mesenchymal stem cells from human endometrium. Biology of Reproduction 80: 1136-45. (>470 citations)

  • Schwab KE, Gargett CE (2007) Co-expression of two perivascular cell markers isolates mesenchymal stem-like cells from human endometrium. Human Reproduction 22: 2903-2911 (>500 citations)

  • Chan RWS, Schwab KE and Gargett CE (2004) Clonogenicity of human endometrial epithelial and stromal cells. Biology of Reproduction 70:1738-1750 (> 650 citiatons)